Table 1 Sequences of primers in this study
Name | Sequence(5′-3′) | Size | Included sgRNAs |
|---|---|---|---|
p1 | 5′-GCTGTCCTCCGCTGCTTTC-3′ 5′-CAGGTCTGCTCCCACTCGTC-3’ | 282 bp | sgRNA1–2 |
p2 | 5′-TCAGACGAGCCCATCAAAGAG − 3′ 5′-AGGGACCCAGCCCAAACA-3’ | 528 bp | sgRNA3–4 |
p3 | 5′-GGGGAGACTGGAGCAGACAA-3′ 5′-GTGCGGGAGCAAAGTGGT-3′ | 560 bp | sgRNA5–6 |
p4 | 5′-AGGGCTGGGCTGGGAAAAAG-3′ 5′-GAGGAAGAGGCTGGGGGAGG-3’ | 3322 bp | sgRNA1–4 |