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Fig. 2 | Lipids in Health and Disease

Fig. 2

From: Perilipin 5 alleviates HCV NS5A-induced lipotoxic injuries in liver

Fig. 2

Plin5 alleviated NS5A-induced lipid peroxidation in liver cells. a and b Livers were isolated and homogenized, and the levels of MDA and 4-hydroxynonenal (4-HNE), two lipid oxidation products, were determined in the homogenate (n = 4). c Primary hepatocytes were isolated from wild-type (Plin5+/+) and Plin5-null (Plin5−/−) mice and infected with control (Ad-Null) and NS5A-expressing (Ad-NS5A) adenoviruses. The expression of NS5A, Plin5 and ATGL in primary hepatocytes was analysed by immunoblotting. d After treatment with 200 μM oleic acid (OA) for 24 h, the ROS levels in hepatocytes with or without NS5A expression were determined by DCFH-DA staining. Scale bar = 50 μm. e and f The levels of MDA and 4-hydroxynonenal (4-HNE) were analysed in primary hepatocytes with or without NS5A expression.**P < 0.01, ***P < 0.001. Data are means ± SEs for triplicate samples for each representative experiment

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