Fig. 1
From: Apolipoprotein-J blocks increased cell injury elicited by ox-LDL via inhibiting ROS-CaMKII pathway
ApoJ overexpression attenuated ox-LDL-induced apoptosis in NRVCs. a. ApoJ expression, after infection by recombinant adenovirus, as determined by western blotting (n = 4/group). b. Cleavage of caspase-3 was enhanced by ox-LDL, but was prevented by ox-LDL (n = 4/group). c. Ox-LDL reduced cell viability, but ApoJ, as determined by MTT assay (n = 8/group), increased it. d. Cardiomyocyte apoptosis as determined by caspase-3/7 activity assay. Apoptosis significantly increased, while ApoJ resisted ox-LDL-induced NRVCs injury (n = 8/group). e. Cell apoptosis as determined by caspase-3/7 activity assay. Apoptosis of control adenovirus-infected cells increased markedly, while cells pretreated with Mn (III) TBAP and KN93 repelled ox-LDL’s detrimental effect (n = 8/group). **P < 0.01 versus control Ad/PBS group; #P < 0.05 versus control Ad/ox-LDL group, ##P < 0.01 versus control Ad/ox-LDL group