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Fig. 4 | Lipids in Health and Disease

Fig. 4

From: Active compound chrysophanol of Cassia tora seeds suppresses heat-induced lipogenesis via inactivation of JNK/p38 MAPK signaling in human sebocytes

Fig. 4

Reduction of pro-inflammatory cytokine levels by CP treatment of heat-shocked human sebocytes. Human sebocytes were serum-starved for 6 h and then incubated for 30 min at 44 °C. Fresh culture medium was added with various concentrations of CP (0–100 μM), followed by incubation for 48 h. a Cytokine levels were detected with an array that included antibodies against 42 different cytokines. Neg, negative control; Pos, positive control. b Three pro-inflammatory cytokines were upregulated by heat treatment (red squares); this effect was mitigated by treatment with CP. c Densitometry analysis of cytokine levels. d Pro-IL-1β protein levels in the whole cell extract and mature IL-1β protein level in the culture supernatant were determined by western blotting, with actin used for normalization of the whole cell lysate. e Mature IL-1β in the culture supernatant was detected by ELISA. Data represent the mean ± SEM (n = 3). *P < 0.05 vs. cells incubated at 37 °C; P < 0.05 vs. cells incubated at 44 °C, (Student’s t test)

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