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Fig. 1 | Lipids in Health and Disease

Fig. 1

From: Generation of an MC3R knock-out pig by CRSPR/Cas9 combined with somatic cell nuclear transfer (SCNT) technology

Fig. 1

The selection of target sites and construction of targeting vectors. a The locations of the target sites in MC3R. sgRNA1 was located between the end of the 3’UTR and the beginning of MC3R, sgRNA2 was in the TM2, sgRNA3 contained part of TM7 and its surrounding sequences, sgRNA4 was in the 5’UTR. b Sequencing results of target sites in the CRISPR/Cas9 targeting vectors. Blue shaded areas indicate the target sequence. c PCR products and measurement of the cleavage efficiencies of the MC3R targeting vectors using the T7E1 assay

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Lipids in Health and Disease

ISSN: 1476-511X