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Table 1 The sgRNA sequences used in this study

From: Generation of an MC3R knock-out pig by CRSPR/Cas9 combined with somatic cell nuclear transfer (SCNT) technology

sgRNA

Sequence (5′ → 3′)

sgRNA1

GCATTCATTGCTCACGGCCG GGG

sgRNA2

GGAGACCATCATGATCGCCG TGG

sgRNA3

GGCTCCGGAAGGCGTAGATC AGG

sgRNA4

CAGGCTAAGGACCGCTCCGG CGG

  1. Note: PAM sequences are underlined
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Lipids in Health and Disease

ISSN: 1476-511X