Table 3 Primers used for qRT-PCR assessment of gene expressions
Gene | Primers | Primer sequence | Tm |
|---|---|---|---|
15-lox-1 | Forward Reverse | 5′ –CAC AGA GAT CCA GTT GCA GA −3 5′- GGC AAG GAG ACA GAA CTC AA-3’ | 58 |
15-lox-2 | Forward Reverse | 5′-GCC TTG AAC TTC TGA CCT CA- 3′ 5′-AGC ATC CAC TGA TTG GAC CT- 3’ | 58 |
Beta-Actin | Forward Reverse | 5-GAT CTC CTT CTG CAT CCT GT-3′ 5′-TGG GCA TCC ACG AAA CTA C- 3’ | 57 |