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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: FoxO3 regulates hepatic triglyceride metabolism via modulation of the expression of sterol regulatory-element binding protein 1c

Fig. 3

FoxO3 gain-of-function results in hepatic steatosis in vitro and in vivo. a-c HepG2 cells were infected with GFP -(Ad-GFP)- or FoxO3-overexpressing adenovirus (Ad-FoxO3) at a multiplicity of infection (MOI) of 50 for 24 h. a Protein analysis of FoxO3 in the adenovirus infection group and control group. b Oil red O staining of HepG2 cells with adenovirus infection. c Cellular triglyceride concentration analysis in HepG2 cells treated with adenovirus. d-h C57BL/6 J male mice (9 weeks old) were injected with Ad-GFP (n = 8) or Ad-FoxO3 (n = 10) through the tail vein at a concentration of 3 × 109 plaque forming units per mouse, fed a chow diet and were housed under regular light/dark cycles for 19 days. (d) Western blot analysis of liver extracts from adenovirus-injected mice. e Oil red O staining of liver sections from representative livers of mice after an overnight fast. f Hepatic triglyceride concentration analysis in mice after an overnight fast. g Gene expression analysis by RT-PCR in livers from Ad-GFP-treated mice (white bars) or Ad-FoxO3-treated mice (red bars). h and j Intraperitoneal glucose tolerance tests (IPGTT) and intraperitoneal insulin tolerance tests (IPITT) were performed on day 8 and day 12 respectively after overnight fast. Blood glucose levels were measured in Ad-GFP-treated mice (black line) or Ad-FoxO3-treated mice (red line) before and 15, 30, 60, and 120 min after injection with 2 g/kg dextrose or 1 U/kg insulin intraperitoneally. i and k Area under the curve (AUC) of blood glucose profiles during IPGTT and IPITT. l Body weight analysis of mice. The data are presented as the mean ± SEM. *P < 0.05 versus Ad-GFP; **P < 0.01 versus Ad-GFP, ***P < 0.001 versus Ad-GFP. FoxO1, Forkhead box class O 1; SREBP1c, sterol regulatory-element binding protein 1c; SCD1, stearyl-coenzyme A desaturase 1; FAS, fatty acid synthase; ACC1, acetyl-CoA carboxylase 1; GPAM, glycerol-3-phosphate acyltransferase; DGAT2, diacylglycerol acyltransferase 2; G6pc, glucose-6-phosphatase; Pck1, phosphoenolpyruvate carboxykinase

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