Fig. 5

FoxO3 transcriptionally stimulates the expression of SREBP1c. a Gene expression analysis of HepG2 cells transduced with FoxO3 siRNA or control siRNA for 48 h. b The protein levels of FoxO3, SREBP1c and SCD1 transfected with FoxO3 siRNA or control siRNA. c Gene expression analysis of HepG2 cells transfected with plasmid. d The protein levels of FoxO3, SREBP1c and SCD1 in HepG2 cells transfected with plasmid. e FoxO3 and SREBP1c protein levels of HepG2 cells treated with adenovirus at different multiplicities of infection (MOIs) for 24 h. f Luciferase (Luc) reporter assays using constructs of the full-length (− 2000/+ 194) SREBP1c gene promoter of mouse. Luc- SREBP1c was co-transfected into HepG2 cells for 24 h with a plasmid vector expressing the constitutively activated form of FoxO3 (Foxo3-TM) or an empty vector or plasmid expressing LXR. ^*P < 0.05 versus control, ^**P < 0.01 versus control, ^***P < 0.001 versus control. FoxO1, Forkhead box class O 1; SREBP1c, sterol regulatory-element binding protein 1c; SCD1, stearyl-coenzyme A desaturase 1; FAS, fatty acid synthase; ACC1, acetyl-CoA carboxylase 1; CD36, fatty acid translocase protein; MTTP, microsomal triglyceride transfer protein; PPARα, peroxisome proliferator-activated receptor; LXR, liver X factor