Table 1 Sequence of primers for quantitative real-time PCR
Gene | Species | Forward primer | Reverse primer |
|---|---|---|---|
FoxO3 | Human | TCAAGGATAAGGGCGACAGC | GGACCCGCATGAATCGACTA |
SREBP-1c | Human | GCGCCTTGACAGGTGAAGTC | GCCAGGGAAGTCACTGTCTTG |
SCD1 | Human | AGCTCATCGTCTGTGGAGCC | GCCACGTCGGGAATTATGAGG |
FAS | Human | GGGATGAACCAGACTGCGTG | TCTGCACTTGGTATTCTGGGT |
ACC1 | Human | ATGTCTGGCTTGCACCTAGTA | CCCCAAAGCGAGTAACAAATTCT |
CD36 | Human | CTTTGGCTTAATGAGACTGGGAC | GCAACAAACATCACCACACCA |
MTTP | Human | ACAAGCTCACGTACTCCACTG | TCCTCCATAGTAAGGCCACATC |
PPARα | Human | TTCGCAATCCATCGGCGAG | CCACAGGATAAGTCACCGAGG |
β-actin | Human | GATGAGATTGGCATGGCTTT | GTCACCTTCACCGTTCCAGT |
FoxO3 | Mouse | CTCACTTTGTCCCAGATCTACG | CTTCATTCTGAACGCGCATG |
SREBP-1c | Mouse | GTGAGCCTGACAAGCAATCA | GGTGCCTACAGAGCAAGAG |
ChREBP | Mouse | AGATGGAGAACCGACGTATCA | ACTGAGCGTGCTGACAAGTC |
SCD1 | Mouse | TTCTTGCGATACACTCTGGTGC | CGGGATTGAATGTTCTTGTCGT |
FAS | Mouse | GCTGCGGAAACTTCAGGAAAT | AGAGACGTGTCACTCCTGGACTT |
ACC1 | Mouse | ATGGGCGGAATGGTCTCTTTC | TGGGGACCTTGTCTTCATCAT |
GPAM | Mouse | ACAGTTGGCACAATAGACGTTT | CCTTCCATTTCAGTGTTGCAGA |
DGAT2 | Mouse | GCGCTACTTCCGAGACTACTT | GGGCCTTATGCCAGGAAACT |
SREBP-2 | Mouse | GCAGCAACGGGACCATTCT | CCCCATGACTAAGTCCTTCAACT |
HMGCS1 | Mouse | AACTGGTGCAGAAATCTCTAGC | GGTTGAATAGCTCAGAACTAGCC |
HMGCR | Mouse | AGCTTGCCCGAATTGTATGTG | TCTGTTGTGAACCATGTGACTTC |
36B4 | Mouse | AGATTCGGGATATGCTGTTGGC | TCGGGTCCTAGACCAGTGTTC |