Fig. 1

Analysis of lipid metabolism, ER stress and mitochondrial function in skeletal muscle of ACE2^−/y mice. a: Relative ACE2 expression level in skeletal muscle of ACE2^−/y mice. b: H&E-stained histology of gastrocnemius muscle in the ACE2^−/y and WT mice (100× H&E). c: Analysis of intramuscular triglyceride concentration in skeletal muscle of ACE2^−/y mice. d: Relative gene expression levels of fatty acid oxidation-related genes (PGC-1α, PPARα, PPARγ, CPT-1α, and MCAD). e: Relative protein levels of lipid-metabolizing (ACCα, SREBP-1c, LXRα and CPT-1α). f: Relative gene expression levels of ER stress-related genes (GRP78, Eif2α, ATF4 and XBP-1). g: Relative protein levels of GRP78, Eif2α, ATF4 and CHOP in the skeletal muscle of ACE2^−/y mice. h: Relative gene expression levels of NDUFB8, SDHB, UQCRC2 and mt-ND1 in the skeletal muscle of ACE2^−/y mice. The data are presented as the mean ± SD of n = 4 independent experiments in ACE2^−/y mice. *P < 0.05 and **P < 0.01 versus WT mice by Student’s t test