Fig. 3

ACE2 regulated ER stress and mitochondrial function in ACE2-overexpressing C2C12 cells and Ad-ACE2-treated db/db mice. a: Relative gene expression levels of ER stress-related genes (GRP78, Eif2α, ATF4 and XBP-1) in ACE2-overexpressing C2C12 cells. b: Relative gene expression levels of BCL-2 and IL-6 in ACE2-overexpressing C2C12 cells. c: H&E-stained histology of gastrocnemius muscle in the Ad-ACE2-treated and Ad-GFP-treated db/db mice (100× H&E). d: Relative gene expression levels of ER stress-related genes (GRP78, Eif2α, ATF4 and XBP-1). e: Relative protein levels of eIF2α and CHOP in the skeletal muscle of Ad-ACE2-treated db/db mice. f: Relative gene expression levels of NDUFB8, SDHB, UQCRC2 and mt-ND1 in the skeletal muscle of Ad-ACE2-treated db/db mice. g: Relative protein levels of NDUFB8 and Bcl-2 in the skeletal muscle of Ad-ACE2-treated db/db mice. The data are presented as the mean ± SD of n = 3 independent experiments in C2C12 cells, n = 3 in Ad-ACE2-treated db/db mice. *P < 0.05 and **P < 0.01 versus Ad-GFP cell or Ad-GFP-treated mice by Student’s t test