Fig. 2

RNA-seq analysis indicated that SULT2B1 is involved in epithelial development. a Schematic diagram of the SULT2B1 gene (blue bars, exons) and the CRISPR/CAS9 gRNA sequence (guide a and b, red). TA cloning results of clone 7 showed that both alleles of the human SULT2B1 gene were modified on exon 2 by the CRISPR/CAS9 system. b The SULT2B1^−/− GES-1 cell line was established by the CRISPR/CAS9 technique. SULT2B1 protein expression was measured by Western blotting analysis in GES-1 cells, SULT2B1^−/− GES-1 cells, and GES-1 cells transfected for 48 h with Ad-GFP or Ad-SULT2B1b. c Among the genes changed by both SULT2B1b overexpression and SULT2B1 deletion, some genes in epithelial development were enriched. The fold changes in these gene levels are shown by the heatmap