Fig. 3

SULT2B1b overexpression promoted gastric epithelial cell proliferation via the PI3K/AKT signaling pathway. a GES-1 cells were treated with Ad-GFP or Ad-SULT2B1b for 48 h and then with IGF-1 (100 ng/mL) or EGF (100 ng/mL) for an additional 24 h. Cell proliferation was detected by the EdU incorporation assay. EdU fluorescence was normalized to that of Hoechst 33342. b, c After transfection with Ad-GFP or Ad-SULT2B1b for 48 h, GES-1 cells were stimulated with IGF-1 (100 ng/mL, b) or EGF (100 ng/mL, c) for 5, 15, 30, 60, or 180 min. AKT phosphorylation levels were detected by Western blotting. The p-AKT/t-AKT ratios are plotted. d After transfection with Ad-GFP or Ad-SULT2B1b for 48 h, GES-1 cells were stimulated with IGF-1 (100 ng/mL) or EGF (100 ng/mL) for 8 h. The mRNA levels of the genes involved in gastric epithelial function were measured by qRT-PCR. * P < 0.05, ** P < 0.01