Table 1 primer sequences used in qRT-PCR
From: Target genes associated with lipid and glucose metabolism in non-alcoholic fatty liver disease
Genes | Accession numbers | Primer sequences(5’to3’) |
|---|---|---|
HMGCR | NM_013134 | aFP:5′-CAATGGCAACAACAGAAGG-3′ |
bRP:5′-CACAAGCACGAGGAAGAC-3’ | ||
IGFBP7 | NM_001013048 | FP:5′-TCACCCAGGTCAGCAAAG-3’ |
RP:5′-GTCACCAGGCAAGAGTTC-3’ | ||
Notch1 | NM_001105721 | FP:5′-GGTGGACATTGACGAGTG-3’ |
RP:5′-GGCATAAGCAGAGGTAGTAG-3’ | ||
ACACB | NM_053922 | FP:5′-CGGCTACTACCTGGACATC-3’ |
RP:5′-TGGAATCGCTTGGCTTGG-3’ | ||
GAPDH | NM_017008 | FP:5′-ATGGTGAAGGTCGGTGTGAACG-3’ |
RP:5′-CGCTCCTGGAAGATGGTGATGG-3’ |