Fig. 5

miR-106b~ 25 deletion effect on macrophages and cytokines. a Relative expression of the miR-106b~ 25 cluster members determined by quantitative real-time PCR in spleen-derived macrophages subjected to M1 (IFNƔ 10 ng/ml and LPS 100 ng/ml) or M2 (IL-4 20 ng/ml) differentiation. M1 and M2 polarization resulted in downregulation of the miR-106b~ 25 cluster members compared to undifferentiated macrophages (*P < 0.05). (B-C)- Relative expression of M1 markers (b) or M2 markers (c) determined by quantitative real-time PCR in spleen-derived lymphocytes isolated from miR-106b~ 25 wild-type or KO mice. d Relative adhesion of spleen-derived macrophages isolated from miR-106b~ 25 wild-type or KO mice and treated with oxidized LDL (20 or 50 μg/ml for 24 h). e Cytokine array of spleen-derived macrophages isolated from miR-106b~ 25 wild-type or KO mice and treated with oxidized LDL (20 μg/ml for 24 h)