Fig. 1

Dose-dependent effects of 7-Ketocholesterol on macrophage lipid content, phenotype and phagocytosis. RAW264.7 were incubated with 7-ketocholesterol at 5 μg/ml (7-KC 5) and 10 μg/ml (7-KC 10) for 24 h, following treatment with 3 μM of GW3965 for 16 h (7-KC 5 + GW) and (7-KC 10 + GW). As control, cells were incubated with ethanol for 24 h following DMSO for 16 h (Control). a Representative Oil Red O staining images, scale bar 20 μm. b Quantification of oil red by determining absorbance at 510 nm after elution with isopropanol. c, d qPCR analysis of mRNA expression levels represented relative to control of the experiments (c) CPT1a and CD36 (d) iNOS and NLRP3. e Net Phagocytosis of 55 μg/ml pHrodo Green E. ecoli bioparticles conjugate for 90 min measured by spectrofluorometer at excitation 485 nm and emission 530 nm. f Representative images of pHrodo uptake capture by fluorescence microscopy, scale bar 20 μm. Data are represented as means ±SEM of 3 independent experiments with n = 3 each. * ≤ 0.05 versus Control; α ≤ 0.05 versus 7-KC 5; β ≤ 0.05 versus 7-KC 10