Fig. 3

CPT1a Knockdown is involved in macrophage phenotype and phagocytosis. RAW264.7 were transduced with adenovirus encoding a short hairpin RNA targeting CPT1a (AdshCPT1a) and a non-target short hairpin RNA (AdshCtrl) for 24 h, as control un-transfected macrophages were used (Control). a, b mRNA expression determined by qPCR of (a) CPT1a and CD36 (b) iNOS and NLRP3. c Representative Oil Red O staining images, scale bar 20 μm. d Quantification of oil red o staining images. e Net Phagocytosis of 55 μg/ml pHrodo Green E. ecoli bioparticles conjugate for 90 min measured by spectrofluorometer at excitation 485 nm and emission 530 nm. f Representative images of pHrodo uptake capture by fluorescence microscopy, scale bar 50 μm. Data are represented as means ±SEM of 4–5 independent experiments with n = 3 each. * ≤ 0.05 versus Control; # ≤ 0.05 versus AdshCtrl