Fig. 4

CPT1a overexpression decreases lipid content and stimulates phagocytosis. RAW264.7 were transfected with either CPT1a CRISPR activation plasmid (CPT1a Crispr) or Control CRISPR activation plasmid (Control Crispr) for 24 h. a, b mRNA expression measure by qPCR of (a) CPT1a and CD36 (b) iNOS and NLRP3. c Representative Oil Red O staining images, scale bar 20 μm. d Quantification of oil red o staining images. e Net Phagocytosis of 55 μg/ml pHrodo Green E. ecoli bioparticles conjugate for 90 min measured by spectrofluorometer at excitation 485 nm and emission 530 nm. f Representative images of pHrodo uptake capture by fluorescence microscopy, scale bar 50 μm. All data are represented as means ±SEM of 3 independent experiments with n = 3 each. * ≤ 0.05 versus Control; # ≤ 0.05 versus Control- Crisp