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Table 1 Primers sequences used for qPCR

From: CPT1a gene expression reverses the inflammatory and anti-phagocytic effect of 7-ketocholesterol in RAW264.7 macrophages

Gene

Forward primer

Reverse primer

GAPDH

TGAAGCAGGCATCTGAGGG

CGAAGGTGGAAGAGTGGGAG

CPT1a

TTTGAATCGGCTCCTAATGG

CCCAAGTATCCACAGGGTCA

CD36

CAGCTCATACATTGCTGTTTATGCATG

GGTACAATCACAGTGTTTTCTACGTGG

iNOS

AGGGAATCTTGGAGCGAGTT

GCAGCCTCTTGTCTTTGACC

NLRP3

Assay ID: qMmuCID0010647. PrimePCR™ SYBR® Green Assay (Bio-Rad).

  1. GAPDH glyceraldehyde 3-phosphate dehydrogenase, CPT1a carnitine palmitoyl transferase 1-a, CD36 cluster of differentiation CD36, iNOS inducible nitric oxide synthase, NLRP3 NOD-like receptor pyrin domain-containing-3
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Lipids in Health and Disease

ISSN: 1476-511X