Fig. 1

Effect of BDNF (10, 50 and 100 ng/ml) pre-treatment (in case of AL/BP) and simultaneous-treatment (in case of STZ/DB) induced cytotoxicity to RIN5F cells in vitro. a) Effect of Pre-treatment with BDNF on AL (6 mM)-induced toxicity to RIN5F cells. All values expressed as mean ± SEM. § P < 0.001 vs untreated control, ***P < 0.001 ₰ P < 0.01 vs alloxan-treated group. **P < 0.01 vs respective untreated control. (B = Brain derived neurotrophic factor). b) Effect of Simultaneous -treatment with BDNF on STZ (20 mM)-induced toxicity to RIN5F cells. All values expressed as mean ± SEM. c) Effect of Simultaneous- treatment with BDNF on doxorubicin (100 ng/ml) induced toxicity to RIN5F cells. All values expressed as mean ± SEM. § P < 0.001 vs untreated control, **P < 0.001 vs doxorubicin-treated group. *P < 0.01 vs respective untreated control. d) Effect of Pre-treatment with BDNF on BP (1.5 mM) - induced toxicity to RIN5F cells. All values expressed as mean ± SEM. *P < 0.001 vs untreated control, # P < 0.05; ₰ P ≤ 0.001 vs BP-treated group. **P ≤ 0.05 § P < 0.01 vs respective untreated control group. (B = Brain derived neurotrophic factor). All the above set of experiments were done in triplicate on three separate occasions (n = 9) and all values expressed as mean ± SEM