Fig. 4

Measurement of lipoxin A4 in RIN5F cell supernatants. a) Effect of BDNF (50 and 100 ng/ml) on the secretion of LXA4 by RIN5F cells in vitro at the end of 24, 48 and 72 h of incubation. All values expressed as mean ± SEM. ϕ P < 0.001, § P < 0.05, ₰ P < 0.01, **P < 0.001 Vs untreated control. BDNF-Brain derived neurotrophic factor. b) Effect of various PUFAs on the secretion of LXA4 by RIN5F cells in vitro that was suppressed by alloxan (6 mM) and STZ (20 mM). All values expressed as mean ± SEM. *P < 0.01 vs untreated control § P < 0.001 and vs toxin-treated group. AA, arachidonic acid; GLA, γ-linolenic acid; EPA, eicosapentaenoic acid; DHA, docosahexaenoic acid. c) Effect of various PUFAs on the secretion of LXA4 by RIN5F cells in vitro that was suppressed by BP (1.5 mM) and DB (100 ng/ml). All values expressed as mean ± SEM. *P < 0.01 vs Untreated control § P < 0.001 and vs toxin -treated group. AA, arachidonic acid; GLA, γ-linolenic acid; EPA, eicosapentaenoic acid; DHA, docosahexaenoic acid. All the above set of experiments were done in triplicate (n = 3) and all values expressed as mean ± SEM.