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Table 4 Summary of analysis of various anti-oxidants in RIN5F treated with Benzopyrene(Pre-treatment)/DB(Sim-treatment) and sub-optimal doses of AA(10 μg/ml) + BDNF(50 ng/ml)

From: PUFAs, BDNF and lipoxin A4 inhibit chemical-induced cytotoxicity of RIN5F cells in vitro and streptozotocin-induced type 2 diabetes mellitus in vivo

SUMMARY OF THE ANALYSIS OF VARIOUS ANTI-OXIDANT ENZYMES IN RIN5F CELLS TREATED WITH DB/BP
CHEMICALGROUPSOD (Units/mg protein)CAT (Mm H2O2/min/mg proteinGST (mM/min/gm protein)GPX (mM/min/gm protein)
DOXORUBICINCONTROL34.6 ± 0.8369 ± 58.644.75 ± 3.4686.8 ± 27.2
DB(100 ng/ml)29.2 ± 1.5§689.1 ± 28§51.65 ± 2.2§1845 ± 69§
AA(10 μg/ml)31.2 ± 0.4414.6 ± 26.537.74 ± 1.8519 ± 14.8
BDNF(50 ng/ml)21 ± 0.9404 ± 29.938.84 ± 2.9601 ± 32.4
AA + BDNF29.5 ± 2.9386 ± 3232.49 ± 2.1515 ± 21.4
DB + AA(10 μg/ml)30.6 ± 1.8323 ± 4139.3 ± 1.2504 ± 5.4
DB + BDNF21 ± 2.8#409 ± 58.241.2 ± 0.8#698 ± 5.9#
DB + AA + BDNF32.25 ± 0.3414.3 ± 5844.4 ± 2.28727 ± 33
BENZOPYRENECONTROL44 ± 1.6515.9 ± 4944.5 ± 0.1791 ± 29
BP(1.5 mM)31.2 ± 1.8§801 ± 61§82.34 ± 1.2 §997 ± 41.3§
AA(10 μg/ml)19 ± 1.2487 ± 5748.9 ± 0.8504 ± 33.3
BDNF(50 ng/ml)18.6 ± 1.7402 ± 24.949 ± 0.62506 ± 39.3
AA + BDNF22 ± 3.1387 ± 38.241.7 ± 0.1567 ± 39.1
BP + AA(10 μg/ml)24 ± 8.2514 ± 39.851.2 ± 1.2798.3 ± 2.6
BP + BDNF31 ± 2.1#498 ± 74.8#48.5 ± 0.2#799.6 ± 3.5#
BP + AA + BDNF39 ± 0.67508 ± 57.152.5 ± 0.08698.1 ± 17.8
  1. Superoxide dismutase (SOD) is expressed as U SOD/mg of protein; Catalase (CAT) is expressed as μM of H2O2 consumed/minute/mg of protein; Glutathione-S-transferase (GST) is expressed as μM conjugate formed/ minute/gm of protein; Glutathione peroxidase (GPX) is expressed as μg of glutathione consumed/minute/gm of protein. ₰ P < 0.05, § P ≤ 0.01 Vs Untreated control and * P < 0.01, # P ≤ 0.05, ** P < 0.001 Vs DB/BP -treated group