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Table 5 Summary of Lipid peroxides and nitric oxide analysis in RIN5F treated with BP(Pre-treatment) /DB(Simultaneous) and suboptimal doses of AA (10 μg/ml) + BDNF(50 ng/ml)

From: PUFAs, BDNF and lipoxin A4 inhibit chemical-induced cytotoxicity of RIN5F cells in vitro and streptozotocin-induced type 2 diabetes mellitus in vivo

SUMMARY OF THE ANALYSIS OF LIPID PEROXIDES AND NITRIC OXIDE LEVELS IN RIN5F CELLS TREATED WITH DB/BP

CHEMICAL

GROUP

LIPID PEROXIDES (μM TMOP)

NITRIC OXIDE (μM Nitrite)

Supernatant Lysates

Supernatant Lysates

DOXORUBICIN

CONTROL

0.43 ± 0.03

1.1 ± 0.03

0.71 ± 0.02

0.9 ± 0.03

DB(100 ng/ml)

0.75 ± 0.02

1.8 ± 0.04

0.91 ± 0.04

1.2 ± 0.03

AA(10 μg/ml)

0.23 ± 0.02

1.1 ± 0.03

0.49 ± 0.02§

1.2 ± 0.02§

BDNF(50 ng/ml)

0.37 ± 0.02

1.3 ± 0.04

0.41 ± 0.01

1.2 ± 0.06

AA + BDNF

0.48 ± 0.01

1.1 ± 0.09

0.39 ± 0.01

1.2 ± 0.04

DB + AA(10 μg/ml)

0.55 ± 0.04#

1.2 ± 0.05

1.02 ± 0.02#

1.1 ± 0.04

DB + BDNF

0.61 ± 0.03§

0.87 ± 0.02§

0.91 ± 0.01§

0.88 ± 0.08§

DB + AA + BDNF

0.59 ± 0.03

1.2 ± 0.06

0.79 ± 0.03

1.1 ± 0.05

BENZOPYRENE

CONTROL

0.58 ± 0.02

0.8 ± 0.02

0.74 ± 0.02

0.98 ± 0.05

BP(1.5 mM)

0.99 ± 0.01

1.6 ± 0.03

0.99 ± 0.03

1.4 ± 0.06

AA(10 μg/ml)

0.61 ± 0.04

1.1 ± 0.03

0.69 ± 0.01

1.11 ± 0.02

BDNF(50 ng/ml)

0.59 ± 0.02

0.89 ± 0.01

0.61 ± 0.03

1.07 ± 0.03

AA + BDNF

0.61 ± 0.01

1.2 ± 0.02

0.72 ± 0.01

1.09 ± 0.04

BP + AA(10 μg/ml)

0.69 ± 0.05#

0.89 ± 0.04#

0.69 ± 0.07#

1.05 ± 0.02#

BP + BDNF

0.72 ± 0.03§

0.7 ± 0.05§

0.71 ± 0.4§

1.1 ± 0.07§

BP + AA + BDNF

0.64 ± 0.06

1.1 ± 0.02

0.79 ± 0.01

1.23 ± 0.03

  1. Lipid peroxides formed are expressed as μmoles of TMOP formed; Nitric oxide formed is expressed as μ moles of nitrite formed. *P < 0.01 Vs Untreated control and § P ≤ 0.05, # P ≤ 0.02, * P < 0.01, ** P < 0.001 Vs DB/BP -treated group