Oleic acid influences the adipogenesis of 3T3-L1 cells via DNA Methylation and may predispose to obesity and obesity-related disorders

Malodobra-Mazur, Malgorzata; Cierzniak, Aneta; Dobosz, Tadeusz

doi:10.1186/s12944-019-1173-6

Lipids in Health and Disease

Table 1 The sequence of primers used for gene expression study and for bisulfate sequencing study

From: Oleic acid influences the adipogenesis of 3T3-L1 cells via DNA Methylation and may predispose to obesity and obesity-related disorders

Gene	Sequence		Location in the gene	Product length [bp]	R^2**
Β-actin	F	CCCAGATCATGTTTGAGACC	2/3^*	53	0,99
Β-actin	R	CTGGATGGCTACGTACATG	2/3^*	53	0,99
Dnmt1	F	ATGAGAGGGAGGAGAAGAGAC	9/10^*	184	0,99
Dnmt1	R	TGCTGCTGGTACTTCAGGTTAG	9/10^*	184	0,99
Dnmt3a	F	CTCAGTGGTGTGTGTGGAGA	2/3^*	196	0,98
Dnmt3a	R	CACTTCCACAGCCTTGCCACT	2/3^*	196	0,98
C/ebpα	F	GACTGGAGTTATGACAAGCTTC	1^*	107	0,99
C/ebpα	R	TGCACACTGCCATTGCACAAG	1^*	107	0,99
C/ebpβ	F	ATCCGGATCAAACGTGGCT	1^*	107	0,99
C/ebpβ	R	AACCCCGCAGGAACATCTTT	1^*	107	0,99
C/ebpδ	F	CGAATCGCTAGTTTCTTTGGG	1^*	75	0,99
C/ebpδ	R	ATAGCTTCTCTCGCAGTCCAG	1^*	75	0,99
Pparγ	F	ATGTCTCACAATGCCATCAGG	4/5^*	98	0,99
Pparγ	R	TCTGGGTTCAGCTGGTCGAT	4/5^*	98	0,99
Insr	F	CCAGGCATCGTGTGAAAATG	6/7^*	160	0,99
Insr	R	CTCTGTCACATTCTGATAAG	6/7^*	160	0,99
Slc2a4	F	CAGGCATCAATGCTGTTTTC	7/8^*	109	0,99
Slc2a4	R	GTGAAGACCGTATTGACCAC	7/8^*	109	0,99
Lep	F	TTCACACACGCAGTCGGTAT	1/2^*	84	0,99
Lep	R	GACAAACTCAGAATGGGGTG	1/2^*	84	0,99
Adipoq	F	AGGAGATGCAGGTCTTCTTG	1/2^*	137	0,99
Adipoq	R	CTGAGCGATACACATAAGC	1/2^*	137	0,99
Ptpn1	F	AAAACCTGACTACCAAGGAG	5/6^*	55	0,99
Ptpn1	R	CATGTGGTGTAGTGGAAATG	5/6^*	55	0,99
Pik3r1	F	GACATCTCAAGGGAAGAAGT	2/3^*	106	0,99
Pik3r1	R	GTGTAAGAGTGTAATCGCCG	2/3^*	106	0,99
Akt1	F	CATTCAGACTGTGGCAGATG	4/5^*	145	0,99
Akt1	R	AAACTCGTTCATGGTCACAC	4/5^*	145	0,99
Fasn	F	TTGATGATTCAGGGAGTGG	4/5^*	126	0,99
Fasn	R	TGCTGCCATCTGTATTGGTGC	4/5^*	126	0,99
Acaca	F	CTTAGAGAGGGGTCAAGTC	14/15^*	149	0,99
Acaca	R	CTTCCACACACGAGCCATTC	14/15^*	149	0,99
Scd1	F	CCCACATGCTCCAAGAGATC	1/2^*	127	0,99
Scd1	R	TCAGGACGGATGTCTTCTTCC	1/2^*	127	0,99
Lpl	F	TCAACTGGATGGAGGAGGAG	3/4^*	103	0,99
Lpl	R	TTGGTCAGACTTCCTGCTAC	3/4^*	103	0,99
Il-6	F	GATACCACTCCCAACAGACC	2/3^*	99	0,98
Il-6	R	GCACAACTCTTTTCTCATTTC	2/3^*	99	0,98
Il-10	F	TAAGGGTTACTTGGGTTGCC	2/3^*	144	0,92
Il-10	R	CGCATCCTGAGGGTCTTCA	2/3^*	144	0,92
C/ebpα	F	TAGAAGGTGTTGGAGTTGATTAGTG	937–1140 bp	193
C/ebpα	R	CCTCTAAATCTCCAACCAAAAAAC	937–1140 bp	193
Pparγ	F	TTAGAGGAAGGTAAATTTTTGATAG	− 1925 - − 1706 bp	219
Pparγ	R	AAACAATCACCCTATCAATCACAC	− 1925 - − 1706 bp	219

^* Number of the exon/ exon junction
^** primers efficiency assessed based on standard curve method

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ISSN: 1476-511X

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