Fig. 2

Analysis of plasma lipoproteins isolated from female rabbits at 15 week after cholesterol diet feeding. An equal volume (2 μL) of each fraction was resolved by electrophoresis in a 1% agarose gel. Lipoproteins were visualized using Fat Red 7B staining, and apolipoproteins were identified by immunoblotting with apoB, apoE, and apoAI Abs (a). An equal volume of each fraction (5 μL) was resolved by electrophoresis by 4 to 20% SDS-PAGE. Apolipoproteins were visualized using Coomassie brilliant blue staining (b). The quantitation of cholesterol and TG contents in lipoprotein fractions of rabbits fed a cholesterol-rich diet for15 weeks (c). TC and TG contents in each density fraction were quantified. Data are expressed as mean ± SD