Fig. 6From: Bioinformatics analysis of vascular RNA-seq data revealed hub genes and pathways in a novel Tibetan minipig atherosclerosis model induced by a high fat/cholesterol dietValidation of hub genes. Validation of TNF, ITGB2, VCAM1, TYROBP, LCP2, SPI1, SQLE, CTSS and PTPN6 gene expression levels using RT-PCR. b Immunohistochemical staining for NF-ĸB p65, VCAM1, and MMP9 in the abdominal aorta, left, scale bar = 250 μm; and right, scale bar = 50 μm. c NF-ĸB p65, VCAM1, and MMP9 protein levels were quantified in atherosclerosis and NC samples. Data are presented as mean ± SEM. **, P < 0.01 versus the NC group. d α-SMA and CD68 immunofluorescence staining and quantitative analysis results in the abdominal aorta. Scale bar = 500 μm. e mRNA expression levels of the top 15 hub genes in human adjacent carotid tissues and atheroma plaque (GSE 43292) data setBack to article page