Fig. 1

Fetuin-A expression in human and mouse adipose tissue and adipocytes. a Total RNA was isolated from human and mouse tissue biopsies and subjected to RT-PCR and agarose gel analysis. b Total RNA was isolated from human primary subcutaneous adipose tissue and visceral adipose tissue adipocytes and mouse 3 T3-L1 cells at various stages of differentiation and subjected to RT-PCR and agarose gel analysis. 15 ng of PCR products were loaded on the agarose gel for all samples, excluding mouse liver and HepG2 cell positive controls, for which only 5 ng were loaded. The gels represent three independent experiments