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Fig. 2 | Lipids in Health and Disease

Fig. 2

From: Dlgap1 negatively regulates browning of white fat cells through effects on cell proliferation and apoptosis

Fig. 2

Molecular effects Dlgap1 overexpression on BAT. a Upper panel, electrophoretogram of amplified Dlgap1 coding sequence; lower panel, identification of the pRIRES2-EGFP-Dlgap1 plasmid using restriction enzyme digestion. P, plasmid; S, single-enzyme digestion of plasmid; D, double-enzyme digestion of plasmid. b Immunofluorescence staining of GAPDH, DLGAP1, LEPTIN, and UCP1 in control and Dlgap1-overexpressing BAT. Bar represents 100 μm. c Results of RT-qPCR of white- and brown-fat marker genes in control and Dlgap1-overexpressing brown fat cells. Top, white-fat marker gene; bottom, brown-fat marker gene. *P < 0.05, ** P < 0.01. *** P < 0.001, **** P < 0.0001. d Western blots of Dlgap1-overexpressing and control brown adipocytes. Data are means ± SD

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Lipids in Health and Disease

ISSN: 1476-511X