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Fig. 4 | Lipids in Health and Disease

Fig. 4

From: Dlgap1 negatively regulates browning of white fat cells through effects on cell proliferation and apoptosis

Fig. 4

Molecular effects of Dlgap1 knockout on white adipocytes. a Upper panel, sgRNA efficiency test; lower panel, cell transfection efficiency test after 24, 48, and 72 h. b Immunofluorescence staining of GAPDH, DLGAP1, LEPTIN, and UCP1 in control and Dlgap1-knockout white adipocytes. Bar represents 100 μm. c Results of RT-qPCR of white and brown-fat marker genes in control and Dlgap1-knockout white adipocytes. Top, white-fat marker gene; bottom, brown-fat marker gene. *P < 0.05, ** P < 0.01. *** P < 0.001, **** P < 0.0001. d Western blots of proteins in Dlgap1-knockout and control cells. Data are means ± SD

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