Phytochemical analysis and Evaluation of hepatoprotective effect of Maytenus royleanus leaves extract against anti-tuberculosis drug induced liver injury in mice

Table 4 Protective effects of MEM on liver antioxidant status

Treatments	CAT (U/min)	POD (U/min)	SOD (U/mg protein)	GSH (nM/g tissue)	GST (μM/mg protein)	GPx (nM/ min/mg protein)	GSR (nM /min/mg protein)	γ-GT (nM /min/mg protein)
Control	1.93 ± 0.16	1.99 ± 0.11	9.23 ± 0.21	40.82 ± 2.07	6.84 ± 0.41	123.20 ± 4.22	211.31 ± 5.22	82.36 ± 3.26
RIPE	0.54 ± 0.11^****	1.11 ± 0.14^****	4.15 ± 0.23 ^****	32.18 ± 1.88^****	3.58 ± 0.45^****	59.28 ± 2.22^****	153.17 ± 4.13^****	61.42 ± 3.45^****
MEM (200 mg/kg) + RIPE	1.83 ± 0.10⁺⁺⁺⁺	1.52 ± 0.13^****+++	6.54 ± 0.17 ^****++++####	35.51 ± 2.13^**	5.35 ± 0.47^****++++	89.70 ± 3.15^****++++####	177.12 ± 4.03^****++++####	68.74 ± 3.87^**** + #
MEM (400 mg/kg) + RIPE	1.89 ± 0.12⁺⁺⁺⁺	1.72 ± 0.14^*++++	8.35 ± 0.19 ^****++++	37.71 ± 2.26⁺⁺⁺	5.56 ± 0.34^***++++	102.07 ± 3.03^****++++	199.29 ± 6.20^**++++	76.38 ± 4.21⁺⁺⁺⁺
Vit B6 + RIPE	2.08 ± 0.12⁺⁺⁺⁺	2.04 ± 0.13⁺⁺⁺⁺	8.12 ± 0.24 ^****++++	36.51 ± 2.05^*+	6.19 ± 0.67⁺⁺⁺⁺	99.24 ± 3.40^****++++	187.30 ± 5.01^****++++	73.54 ± 3.17^**++++
Vit B6	1.88 ± 0.14⁺⁺⁺⁺	2.18 ± 0.15⁺⁺⁺⁺	11.32 ± 0.18^****++++	41.46 ± 2.20⁺⁺⁺⁺	6.36 ± 0.46⁺⁺⁺⁺	118.50 ± 3.21⁺⁺⁺⁺	209.40 ± 6.05^a++++	84.26 ± 4.12^a++++
MEM (400 mg/kg)	2.42 ± 0.17^**++++	1.83 ± 0.17⁺⁺⁺⁺	10.36 ± 0.23 ^**++++	42.39 ± 1.67⁺⁺⁺⁺	5.62 ± 0.48^**++++	119.25 ± 3.59⁺⁺⁺⁺	201.33 ± 5.02^*++++	85.65 ± 4.65^a++++

Values are Mean ± SD (06 number). RIPE, Rifampicin (13.5 mg/kg), Isoniazid (6.75 mg/kg), Pyrazinamide (36.0 mg/kg) and Ethambutol (24.8 mg/kg). MEM, M. royleanus leaves methanol extract. Vit B6, Vitamin B6. *, **, *** indicate significance from the control group at p < 0.05, p < 0.01 and p < 0.0001 probability level, +, ++, +++ indicate significance from the RIPE group at p < 0.05, p < 0.01 and p < 0.0001, while ### indicate significance of MEM (400 mg/kg) + RIPE group vs MEM (200 mg/kg) + RIPE group at p < 0.0001 probability level (One-way ANOVA followed by Tukey’s multiple comparison tests)

ISSN: 1476-511X