Phytochemical analysis and Evaluation of hepatoprotective effect of Maytenus royleanus leaves extract against anti-tuberculosis drug induced liver injury in mice

Table 5 Protective effects of MEM on liver protein and oxidative stress markers

Treatments	Protein (μg/mg tissue)	TBARS (nM of MDA/mg protein)	H₂O₂ (nM/min /mg tissue)
Control	1.32 ± 0.16	6.09 ± 1.30	5.38 ± 0.42
RIPE	0.73 ± 0.12^****	18.65 ± 3.72^****	8.88 ± 1.04^****
MEM (200 mg/kg) + RIPE	1.10 ± 0.15⁺⁺	13.65 ± 1.82^****++#	6.98 ± 0.80^**+++
MEM (400 mg/kg) + RIPE	1.21 ± 0.13⁺⁺⁺⁺	9.78 ± 1.31^*++++	5.78 ± 0.58⁺⁺⁺⁺
Vit B6+ RIPE	1.16 ± 0.18⁺⁺⁺	9.43 ± 1.55^c++++	6.54 ± 0.66⁺⁺⁺⁺
Vit B6	1.41 ± 0.18⁺⁺⁺⁺	5.80 ± 0.80^e++++	4.95 ± 0.60⁺⁺⁺⁺
MEM (400 mg/kg) alone	1.38 ± 0.15⁺⁺⁺⁺	7.07 ± 1.79^d++++	5.44 ± 0.52⁺⁺⁺⁺

Values are Mean ± SD (06 number). RIPE, Rifampicin (13.5 mg/kg), Isoniazid (6.75 mg/kg), Pyrazinamide (36.0 mg/kg) and Ethambutol (24.8 mg/kg). MEM, M. royleanus leaves methanol extract. Vit B6, Vitamin B6. *, **, *** indicate significance from the control group at p < 0.05, p < 0.01 and p < 0.0001 probability level, +, ++, +++ indicate significance from the RIPE group at p < 0.05, p < 0.01 and p < 0.0001, while ### indicate significance of MEM (400 mg/kg) + RIPE group vs MEM (200 mg/kg) + RIPE group at p < 0.0001 probability level (One-way ANOVA followed by Tukey’s multiple comparison tests)

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