Skip to main content
Fig. 1 | Lipids in Health and Disease

Fig. 1

From: Orotic acid-treated hepatocellular carcinoma cells resist steatosis by modification of fatty acid metabolism

Fig. 1

Nile red staining, triacylglycerol (TAG) measurements and propidium iodide (PI) staining. HepG2 cells were treated with either orotic acid (OA; 500 μM), lipopolysaccharide (LPS; 50 ng/ml) or both, and fixed after 5 days. Cellular lipids were stained with Nile red stain, and cells were observed with a fluorescence confocal microscope (a). Scale bar 20 μm, DAPI = nuclei. The total intensity (number of pixels × mean intensity) of Nile red stain signal was quantified with the ZEN 2009 software (b). Quantification results were normalized by dividing the treatment sample values by control measurements. Results are presented as mean % of control measurements + SEM and counted from five independent experiments, each including at least 10 images per sample. Total TAG levels in OA-treated cells were measured by colorimetric assay (c). The amount of TAG (mg) was normalized to cell count. Results are presented as mean + SEM and counted from three independent experiments. Viability of OA-treated cells was evaluated by PI staining (d). OA-treated cells were stained with PI stain and observed with a fluorescence confocal microscope. PI-positive cells were manually counted from three independent experiments, each including at least 1300 cells per sample. Results are presented as mean % of PI-positive cells from total cell count + SEM

Back to article page