Table 1 Primer sequence for qRT-PCR in this study
From: MiR-30b-5p regulates the lipid metabolism by targeting PPARGC1A in Huh-7 cell line
Gene | Primer/ Sequences of Product ID of Primer |
|---|---|
hsa-miR-30b-5p | F: CD201–0037 (TIANGEN, Beijing, China) |
R: R7015 (TIANGEN, Beijing, China) | |
U6 | F: CD201–0145 (TIANGEN, Beijing, China) |
R: R7015 (TIANGEN, Beijing, China) | |
PPARGC1A | F: 5′-CCTGTGATGCTTTTGCTGCTCTTG-3′ |
R: 5′-AAACTATCAAAATCCAGAGAGTCA-3′ | |
β-actin | F: 5′-TGGACTTCGAGCAAGAGATG-3′ |
R: 5′-GAAGGAAGGCTGGAAGAGTG-3′ |