Table 1 Primers used in this study
From: MiR-9-5p promotes rabbit preadipocyte differentiation by suppressing leptin gene expression
Gene name | Primer sequence (5′-3′) | (Tm/°C) | (Product size/bp) |
|---|---|---|---|
leptin | CTGTGCCCATGCGGAAAGTC | 61.4 | 104 |
AGTCCAAACCGACGACCCTC | Â | Â | |
β-actin | GGAGATCGTGCGGGACAT | 61.4 | 318 |
GTTGAAGGTGGTCTCGTGGAT | Â | Â | |
PPARγ | GAGGACATCCAGGACAACC | 61 | 168 |
GTCCGTCTCCGTCTTCTTT | Â | Â | |
FABP4 | GGCCAGGAATTTGATGAAGTC | 61.4 | 140 |
AGTTTATCGCCCTCCCGTT | Â | Â | |
C/EBPα | GCGGGAACGAACAACAT | 64 | 172 |
GGCGGTCATTGTCACTGGTC | Â | Â | |
miR-9-5p mimic | UCUUUGGUUAUCUAGCUGUAUGA AUACAGCUAGAUAACCAAAGAUU | Â | Â |
Negative Control (NC) | UUCUCCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT | Â | Â |
inhibitor-9-5p | UCAUACAGCUAGAUAACCAAAGA | Â | Â |
inhibitor NC | CAGUACUUUUGUGUAGUACAA | Â | Â |
si-leptin-1 | CCACAAUGGACCAGACGUUTT AACGUCUGGUCCAUUGUGGTT | Â | Â |
si-leptin-2 | CCAUUGUCACCAGGAUCAGTT CUGAUCCUGGUGACAAUGGTT | Â | Â |
si-leptin-3 | CCGAAAUGUGAUCCAAAUATT UAUUUGGAUCACAUUUCGGTT | Â | Â |
Primers for gene 3’UTR | ATGGGTAAGTACATCAAGAG |  |  |
cloning leptin | GAGGTCCGAAGACTCATTT | Â | Â |