Fig. 3
FA exposed HUVEC soluble mediator release. Comparison of soluble mediator release by HUVEC exposed to OA and its metabolites. Conditioned media was collected after 24 h exposure to OA-BSA, 2-OH OA (Na+), or 12-OH OA-EtOH or their equivalent vehicle controls. Mediators were measured by ELISA, as described in methods. a PGF2α release. b ET-1 release. Each value is the concentration as a percentage of each group’s vehicle control, mean ± S.E. of 3 trials. Comparisons between groups by dose are done by two-way ANOVA. ** = p < 0.005, *** = p < 0.001, **** = p < 0.0001