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Table 1 HUVEC gene expression changes

From: Oleic acid and derivatives affect human endothelial cell mitochondrial function and vasoactive mediator production

[OA] (μM)

0

100

250

500

1000

Gene

Mean ± SEM

n

Mean ± SEM

n

Mean ± SEM

n

Mean ± SEM

n

mean ± SEM

n

EDN1

1.15 ± 0.57

2

1.18 ± 0.48

2

1.05 ± 0.39

2

0.99 ± 0.46

2

0.71 ± 0.18

2

COX2

1.02 ± 0.21

2

0.95 ± 0.05

2

0.8 ± 0.06

2

0.67 ± 0.02

2

0.21 ± 0.1 *

2

NOS3

1.01 ± 0.12

2

1.15 ± 0.29

2

1.14 ± 0.21

2

1.3 ± 0.41

2

0.79 ± 0.6

2

ICAM

1.01 ± 0.15

2

1.04 ± 0.03

2

1.1 ± 0.05

2

1.18 ± 0.06

2

0.39 ± 0.31

2

[12-OH OA] (μM)

0

100

  

500

  

Gene

Mean ± SEM

n

Mean ± SEM

n

  

Mean ± SEM

n

  

EDN1

1.05 ± 0.19

3

0.83 ± 0.35

3

  

0.98 ± 0.22

3

  

COX2

1.21 ± 0.4

3

0.16 ± 0.08 *

3

  

0.05 ± 0.02 *

3

  

NOS3

1.09 ± 0.26

3

1.58 ± 0.49

3

  

0.98 ± 0.36

3

  

ICAM

1.46 ± 0.5

3

0.75 ± 0.22 *

3

  

0.22 ± 0.05 *

3

  
  1. Abbreviations: Edn1 Endothelin-1, Cox2 Cyclooxygenase-2, Nos3 endothelial nitric oxide synthase, Icam1 Intercellular Adhesion Molecule 1. Each value is the mean fold change ± S.E.M.. Mean expression values of markers are compared for significant exposure effects relative to vehicle-exposed group and normalized to beta actin (Actb) levels (* = p < 0.05)