Fig. 3
From: High fat diet altered cardiac metabolic gene profile in Psammomys obesus gerbils
Myocardial activation of apoptotic processes assessed by terminal deoxynucleotidyl transferase biotin-dUTP nick-end labeling (TUNEL) in Psammomys obesus gerbils fed with normal diet or high fat diet during 16 weeks. Myocardial sections were obtained at 400-fold (a; Scale bars: 50 μm). Cardiac apoptotic rate (in percentage; b) was evaluated as the ratio between the number of terminal deoxynucleotidyl transferase biotin-dUTP nick-end labeling-positive cardiomyocytes (brown nuclei mentioned by arrows) and the total number of cardiomyocytes (brown + blue nuclei) in 16-week normal (n = 10; yellow bars) and high fat diet (n = 10; blue bars)-fed animals. Data are expressed as mean ± SEM.***P < 0.001 versus the normal diet fed animals