Fig. 2

APOA1 RNA is upregulated by ethanol and requires RNA polymerase II activity. Relative RNA expression levels were determined by RT-qPCR as described in Methods. HepG2 were cultured to ~ 80% confluence on six well plates before treating with ethanol. a HepG2 cells were exposed to 100 mM ethanol and/or α-amanitin as per Materials and Methods. b and c Relative RNA expression levels were determined by RT-PCR as described in Methods. Dose response curve of APOA1 RNA at the indicated final concentrations of ethanol in the medium. The error bars represent the SD from the mean of five assays of an individual experiment. *Significant difference, ethanol treatment versus untreated control (1 way ANOVA with multiple comparisons, n = 5; *P < 0.05, **P < 0.01, ***P < 0.001 vs. control)