Fig. 1

Effects of H₂O₂ and AAV9 vectors on NRVMs. a-c Western blotting and quantified cytosolic or nuclear protein levels of IκBα and p65 in NRVMs exposed to 100 μM H₂O₂ for 0, 15, 30, and 60 min (n = 3, *P < 0.05 and **P < 0.01 vs. H₂O₂ (0 min)). d-e Western blotting and quantified total protein levels of p-p65 and p65 in NRVMs subjected to 100 μM H₂O₂ for 0, 15, 30, 60 min (n = 3, *P < 0.05 and **P < 0.01 vs. H₂O₂ (0 min)). f The green fluorescence intensity of GFP in NRVMs (scale bar: 250 μm). g-i Western blotting to detect the protein expression of IκBα and GFP. (n = 3, ** P < 0.01 vs. Control)