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Fig. 4 | Lipids in Health and Disease

Fig. 4

From: Overexpression of IκBα in cardiomyocytes alleviates hydrogen peroxide-induced apoptosis and autophagy by inhibiting NF-κB activation

Fig. 4

IκBα suppressed H2O2-induced NF-κB activation and autophagy in NRVMs. a The distribution of NF-κB p65 (red fluorescence) was detected using immunofluorescence (scale bar: 25 μm). The results showed that IκBα or PDTC inhibited H2O2-induced p65 nuclear translocation. b Western blotting and quantified cytosolic or nuclear protein levels of IκBα and p65 in NRVMs exposed to 100 μM H2O2. The results showed that IκBα transduction maintained the cytosolic IκBα level and suppressed p65 nuclear translocation (n = 3, ** P < 0.01 vs. Control, ##P < 0.01 vs. H2O2 group). c Western blotting and quantified total protein levels of p-p65/p65, Beclin-1 and LC3-II/LC3-I in NRVMs exposed to 100 μM H2O2. The findings showed that IκBα or PDTC decreased the p-p65/p65 ratio, Beclin-1 expression and the LC3-II/LC3-I ratio after H2O2 stimulation. (n = 3, ** P < 0.01 vs. Control, ##P < 0.01 vs. H2O2 group)

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