Fig. 1

AdipoRon attenuates palmitate induced ROS production. L6 cells were incubated with 0.1 mM palmitate (PA) or vehicle control (Con) and were also treated with 35 μM AdipoRon as indicated for various timepoints as indicated following which ROS was measured using a) CellROX green live cell imaging (shown as mean ± 95% confidence intervals for 150 cells) or b) using DCF-DA. Graphs are displayed as boxplots; a = P < 0.05 vs 1 h Con, b = P < 0.05 vs 1 h Con+AdipoRon, c = P < 0.05 vs 1 h PA, d = P < 0.05 vs 2 h Con, e = P < 0.05 vs 2 h Con+AdipoRon, f = P < 0.05 vs 2 h PA, g = P < 0.05 vs 4 h Con, h = P < 0.05 vs 4 h Con+AdipoRon, i = P < 0.05 vs 4 h PA, red bolded letters indicate P values which were rendered nonsignificant > 0.05 after FDR calculation, n = 4–5