Fig. 4
From: An adiponectin-S1P autocrine axis protects skeletal muscle cells from palmitate-induced cell death
Pharmacological manipulation of S1P significantly alters palmitate-induced ROS production and cell death. L6 cells were incubated with 0.1 mM palmitate (PA) or vehicle control (Con) and were also treated with either 35 μM AdipoRon, 5 μM SKI-II, or 5 μM THI for either 2 or 4 h following which a) ROS was measured using DCF-DA. Additionally after 24 h cell death (shown as % toxicity) was measured using b) MTT or C) LDH. Graphs are displayed as boxplots; a = P < 0.05 vs corresponding PA, b = P < 0.05 vs corresponding PA + AdipoRon, c = P < 0.05 vs corresponding PA + SKI-II, d = P < 0.05 vs corresponding PA + THI, n = 4