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Table 1 Elution programme of liquid chromatography

From: Lipidomics profiling of skin surface lipids in senile pruritus

Time (min) Flow (mL/min) A% B%
0 0.5 90 10
0.5 0.5 90 10
11 0.5 0 100
17 0.5 0 100
17.1 0.5 90 10
20 0.5 90 10
  1. mobile phase A:50% water + 50%ACN (Acetonitrile) + 10 mM ammonium formate
  2. mobile phase B: 10% ACN + 90% IPA (Isopropanol) + 10 mM ammonium formate