Table 2 Oligonucleotide primers used for quantitative real-time polymerase chain reaction
From: Two weeks of western diet disrupts liver molecular markers of cholesterol metabolism in rats
| Gene name NCBI | Alias | Oligo FWD | Oligo REV |
Sonde UPL |
|---|---|---|---|---|
| Abcg5 | ABCG5 | cggagagttggtgttctgtg | caccgatgtcaagtccatgt | 82 |
| Abcg8 | ABCG8 | cagatgctggctatcataggg | ctgatttcatcttgccacca | 13 |
| Acat2 | ACAT-2 | agaagtccttcagagagccaaa | cactggcttgtcgagtagga | 50 |
| Acaca | ACC | acagagatggtggctgatgtc | gatccccatggcaatctg | 4 |
| Actb | Actb (control) | cccgcgagtacaaccttct | cgtcatccatggcgaact | 17 |
| Mlxipl | ChREBP | aatcccagcccctacacc | ctgggaggagccaatgtg | 10 |
| Gapdh | Gapdh (control) | ccctcaagattgtcagcaatg | agttgtcatggatgaccttgg | 73 |
| Hmgcr | HMGCoAR | caaccttctacctcagcaagc | acagtgccacacacaattcg | 80 |
| Hprt | Hprt (control) | gaccggttctgtcatgtcg | acctggttcatcatcactaatcac | 95 |
| Ldlr | LDL-R | tgctactggccaaggacat | ctgggtggtcggtacagtg | 16 |
| Lrp1 | LRP-1 | aatcgagggcaagatgacac | ccagtctgtccagtacatccac | 81 |
| Pcsk9 | PCSK9 | ccagtgtccacgcttcct | gatgccatgctccttgattt | 1 |
| Scarb1 | SR-B1 | ggtgcccatcatttaccaac | gcgagccctttttactacca | 71 |
| Srebf2 | SREBP2 | gtgcagacagtcgctacacc | aatctgaggctgaaccagga | 62 |