Fig. 1
Sterol analysis of the PLBWT and PLB4 brain. The relative abundance of each sterol identified in the cortex (A), hippocampus (B), and hypothalamus (C) was normalised to D7-cholesterol as the internal standard. Annotation of the major sterol species were identified from MS daughter ion fragmentation scans. Data represented as average ± S.E.M (n = 6). The normalised abundance (arbitrary units) refers to the normalised total ion count for individual molecular species. An unpaired, two-tailed Student’s t-test with Welch’s correction was performed to determine statistically significant differences between PLBWT and PLB4 data. * P ≤ 0.05, ** P ≤ 0.01. Cortex: all p ≥ 0.05; hippocampus: dehydrocholesterol P = 0.0113, didehydrocholesterol P = 0.0099, cholesterol P = 0.0151; hypothalamus: cholesterol P = 0.0326