Fig. 3
Decreased IKATP density in NRCMs treated with L5. a, Representative traces of IKATP in NRCMs treated with Pin-baseline (20 μM pinacidil, IKATP opener), Pin+L5 (7.5 μg/mL), Pin-washout, and Pin+Gli (100 μM glibenclamide, IKATP blocker). b, Time-course analysis showing the effect of L5 on IKATP. c, The perfusion of NRCMs with L5 reduced IKATP, which partially recovered after 5 min of washout (n = 5 per group, **P < 0.01,*P < 0.05 vs. Pin-baseline, #P < 0.05 vs. Pin+L5). d, The overnight incubation of NRCMs with L5 decreased the expression level of Kir6.2, but no change was seen after overnight incubation with L1, n = 3 per group. *P < 0.05 vs. PBS