Fig. 3

C6-ceramide induces phosphorylation of JAK2 and STAT3 in BEAS-2B cells. After serum starvation for 24 h, BEAS-2B cells were treated with C6-ceramide (10 μM) for 24 h. (a) JAK2 and p-JAK2 expression was examined by Western blotting, and relative quantification of the p-JAK2/JAK2 expression was determined by densitometric analysis of the blots. (b) STAT3 and p-STAT3 expression was examined by Western blotting, and relative quantification of the p-STAT3/STAT3 expression was determined by densitometric analysis of the blots. n = 3 per group. ^*P < 0.05 vs controls that were cultured in the absence of C6-ceramide