Skip to main content
Fig. 2 | Lipids in Health and Disease

Fig. 2

From: Imaging the rapid yet transient accumulation of regulatory lipids, lipid kinases, and protein kinases during membrane fusion, at sites of exocytosis of MMP-9 in MCF-7 cells

Fig. 2

TIRF images of cotransfected MCF-7 cells containing MMP9-GFP and red fluorescent-labeled PIP2-sensor (a–d) or DAG-sensor (e–h). Exocytic fusion events analysis in the MMP9-GFP (green channel; a, e) and the corresponding area in the red channel with the PIP2-sensor (b) or DAG-sensor (f), in PMA-stimulated cells. Time-lapse snapshots (with timepoints) of fusion events are shown, along with average normalized fluorescence intensity trajectories in the green (c & g) and red channels (d & h). The SE of the data are plotted as gray lines. Number of fusion events (N) used in the averaging are N = 25 for PIP2-sensor and 33 for DAG2-sensor. Dashed vertical line corresponds to the fusion event (0 s) of MMP9-GFP in the green channel, which was time-aligned to the red channel

Back to article page

Lipids in Health and Disease

ISSN: 1476-511X