Fig. 2

Generation of mutant CBS alleles by injecting rabbit zygotes with CRISPR/Cas9. a Types of mutations in the CBS alleles in founder rabbits. The WT and donor ssODN template sequences are shown at the top. Silent mutations, point mutations, substitutions (>) and insertions (+) are labeled with green letters, red underlined letters, black lowercase letters and blue letters respectively. The deletions (∆) are indicated on the right of the respective allele. b Theoretical amino acid sequences of the founder rabbits. The amino acid at 307 is shown with a black arrow. Deletions (∆) and substitutions (>) are shown to the right of each allele